mouse wnt5a antibody  (Bioss)

Journal: Cell Genomics

Article Title: Single-cell and spatial transcriptomic profiling revealed niche interactions sustaining growth of endometriotic lesions

doi: 10.1016/j.xgen.2024.100737

Figure Lengend Snippet:

Article Snippet: mouse monoclonal anti-WNT5A , Santa Cruz Biotechnology , Cat#sc-365370; RRID:AB_10846090.

Techniques: Recombinant, Red Blood Cell Lysis, Blocking Assay, RNA Sequencing, Gene Expression, Control, Software

Journal: Science Advances

Article Title: A fibronectin gradient remodels mixed-phase mesoderm

doi: 10.1126/sciadv.adl6366

Figure Lengend Snippet: ( A ) Transverse sections of 21 som. CD1 embryos at forelimb anterior and posterior regions. Sections were stained with 4′,6-diamidino-2-phenylindole (DAPI; cyan) anti-YAP (green) and anti-fibronectin antibody (red). ( B ) Relative fibronectin fluorescence intensity versus YAP nuclear/cytoplasmic ratio of 20 to 21 som. CD1 ( n = 3) forelimbs. PCC, Pearson correlation coefficient. ( C ) YAP nuclear/cytoplasmic ratio of 20 to 21 som. CD1 embryos ( n = 3) at forelimb anterior and posterior regions (two-tailed unpaired Student’s t test, **** P < 0.0001). ( D ) Transverse sections of 20 som. T:Cre; Yap f/f ; Taz f/+ embryos at forelimb anterior and posterior regions. Sections were stained with DAPI (cyan) anti-YAP (green) and anti-fibronectin antibody (red). ( E ) Relative fibronectin fluorescence intensity in 20 to 21 som. Yap f/+ ; Taz f/+ ( n = 5) versus T:Cre; Yap f/f ; Taz f/+ ( n = 3) embryos at forelimb anterior and posterior regions (two-tailed unpaired Student’s t test, ** P < 0.01). Error bars indicate SD. ( F ) Limb bud shape change among T:Cre; Yap f/f ; Taz f/+ embryos from 20 to 25 som. stage reconstructed from OPT. ( G ) Schematic model representing the WNT5A-YAP-FN signaling pathway that establishes tissue stiffness and orchestrates cell movements to drive limb bud shape change. ( H ) Variations in stiffness gradient geometry correspond to patterns of morphogenetic cell movements in the limb bud (top) and mandibular arch (bottom).

Article Snippet: Primary antibodies included fibronectin (1:100; Abcam, lot ab2413), pHH3 (1:250; Cell Signaling Technology, lot 9706), N-cadherin (1:200; BD Biosciences, lot 610920), β-catenin (1:200; Abcam, lot ab59430), Vangl2 (1:200; Sigma-Aldrich, lot HPA027043), p-SAPK/JNK (1:200; Cell Signaling Technology, lot 9255), YAP (1:200; Abcam, lot ab56701), FAK (1:200; BD Biosciences, lot 610087), phospho-YAP (1:200; Cell Signaling Technology, lot 4911), and WNT5A (1:200; R&D Systems, lot MAB645).

Techniques: Staining, Fluorescence, Two Tailed Test